影音先锋中文字幕无码-精品一区二区三区av天堂-波多野结衣小视频-欧美视频亚洲-性色网站-久久国产中文字幕-麻婆豆传媒一区二区三区-精品乱码一区二区三区-天天狠天天透天干天干-国内精品写真在线观看-国产a√-99国产精品久久不卡毛片-在线免费播放av-日韩福利在线播放-国产无遮挡裸露视频免费-交视频在线播放-国产成人最新三级在线视频-免费三片在线观看网站v888-亚洲偷自拍国综合色帝国-国产一级特黄毛片

    恒遠生物 專業致力于打造國際民族品牌，提供全面的、創新的、優質的、便捷的科研產品和服務。目前，我們自主研發的重組蛋白、抗體、ELISA檢測試劑盒得到廣大科研用戶的認可，并且多次發表于國際期刊，進一步推動了民族品牌國際化的傳播。

 五月，大家的論文寫得怎么樣了？

【文獻標題】

二、產品原文引用

Cytokine Assays

After conjugation, PLGA and Chitosan nanoparticles were precipitated and the free amounts of rHCA59 protein were determined by a BCA protein assay kit. It was found that 89% and 71% of rHCA59 were conjugated to PLGA and Chitosan, respectively. In other words, a total of 1.78 mg of protein bound to PLGA, and 1.42 mg of protein bound to Chitosan.A scanning electron microscopy was used to identify the size and morphology of PLGA and Chitosan nanoparticles. The SEM results showed that both PLGA and Chitosan nanoparticles’ appearances were smooth. The size of rHCA59-PLGA NPs was from 180 nm to 402 nm (Figure 1A) and the size of rHCA59-CS NPs was from 200 nm to 500 nm (Figure 1B).

Notes: (1): PBS group (blank control). (2): pET-32a protein group. (3): PBS-PLGA group. (4): PBS-CS group. (5): PBS-CFA group. (6): rHCA59 group. (7): rHCA59-PLGA group. (8): rHCA59-CS group. (9): rHCA59-CFA group. (10): rHCA59-CS-PLGA group. (A) IL-12. (B) IL-17. (C) IFN-γ. (D) IL-4. (E) TGF-β. Data are representative of triplicate independent experiments (*p < 0.05, **p < 0.01, and ***p < 0.001). Abbreviations: PLGA, poly-D,L-lactide-co-glycolide; CS, Chitosan; CFA, complete Freund’s adjuvant; IL, interleukin; ELISA, enzyme linked immunosorbent assay

Notes: rHCA59 loaded nanoparticles could affect the proportions of CD4+ T cells and CD8+ T cells in immunized mice. The percentages of CD4+ T cells (A1–A10) and CD8+ T cells (B1–B10) in ten groups were investigated by flow cytometry on day 14 after immunization. (C and D) Different treatments affected the proportions of CD4+ T cells and CD8+ T cells. (A1 and B1) PBS group (blank control). (A2 and B2) PBS-CFA group. (A3 and B3) PBS-PLGA group. (A4 and B4) PBS-CS group. (A5 and B5) pET-32a protein group. (A6 and B6) rHCA59 group. (A7 and B7) rHCA59-CFA group. (A8 and B8) rHCA59-CS group. (A9 and B9) rHCA59-PLGA group. (A10 and B10) rHCA59-CS-PLGA group. The results shown here are from three independent experiments (*p < 0.05, **p < 0.01, and ***p < 0.001). Abbreviations: CD4, cluster of differentiation 4; CD8, cluster of differentiation 8; T cells, thymus cells; PLGA, poly-D,L-lactide-co-glycolide; CS, Chitosan; CFA, complete Freund’s adjuvant.